👨🔬Научните изследвания до този момент не са успяли да открият никакви хистологични и физиологични доказателства за съществуването както на енергията „чи“, така и на акупунктурните точки и меридианите. Нито един и от подръжниците на акупунктурата досега не е успял да открие тази „витална енергия“ или да формулира научно съгласувана теория, определяща вида, произхода и влиянето й [1,29]. Без научно определение на енергия „чи“ няма как да съществува и основа, върху която акупунктурата да бъде приемана и използвана като медицинска интервенция [2]. Някои от поддръжниците на акупунктурата изразяват хипотези за ефективността на акупунктурата, базиращи се на физически механизми като освобождаване на естествени болкоуспокояващи химикали, отпускане на мускули или инхибиране на проводимостта на болката чрез противовъзпалително действие. Въпреки че тези механизми звучат доста по-праводподобно от несъществуващата енергия „чи“, те си остават много спекулативни и не могат да обяснят твърденията за „лечителното“ действие на акупунктурата и съществуването на акупунктурни точки [2,11].
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